Composite
SDM GFP
Part:BBa_K2477003:Design
Designed by: Jacob Premo Group: iGEM17_UMaryland (2017-10-25)
GFP with PAM site (BBa_K2477000) expression system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 705
Design Notes
We used site directed mutagenesis.
Source
GFP in iGEM kit (E0040)